Figure 3

Administration of AAV GCG-EGFP does not alter body weight or glucose homeostasis. Four hour fasted (A) body weight and (B) blood glucose, before and after intraperitoneal administration of several doses of AAV GCG-EGFP from 10¹⁰ to 10¹³ vg on day 0. (C) Serum AST and ALT levels 2 weeks post intraperitoneal injection of AAV. (D) OGTT was performed following a dextrose load of 1.5 g/kg body weight after a 4 hour fast, 10 weeks after AAV administration. An arginine tolerance test was performed via an arginine injection of 2 g/kg body weight after a 4 hour fast, 13 weeks after AAV injection, with (E) blood glucose and (F) plasma glucagon levels measured at different timepoints following arginine administration. (G) Plasma insulin levels after overnight fasting and (H) plasma glucagon levels after overnight fasting and in the re-fed state (2 hr ad libitum feeding) 23 weeks after AAV injection. AST: aspartate aminotransferase; ALT: alanine aminotransferase; vg: viral genomes. Data are expressed as mean ± SEM. Statistical analysis was performed using Kruskal-Wallis Test or two-way ANOVA (no significant differences observed among groups). A, B, D and E, n = 4 in PBS, 10¹⁰ and 10¹³ groups, and n = 5 in 10¹¹ and 10¹² groups.