Figure 4

Intracellular Ca²⁺ pattern in EGFP positive and EGFP negative α-cells in response to glucose and adrenaline. (A) Bright field and (B) fluorescent EGFP signals in dispersed islet cells. (C) Raw Ca²⁺ traces from single EGFP⁺ (green) and EGFP⁻ (grey) α-cells in response to low glucose (0.5 mM), high glucose (11 mM) and adrenaline (5 µM). (D) Summary statistics for the cell types sorted by cluster. A total of 33 EGFP⁺ and 27 EGFP⁻ α-cells from 5 AAV-treated mice were imaged.