Figure 7

4q35 gene expression is not modulated in FSHD hiPSCs compared to controls. Gene expression quantified by RT-qPCR in control FSHD1 hIPSC (A, N; respectively - see Table 1). Each measure represents the average fold-change expression of six independent assays (Biological triplicate in technical RT duplicate) normalized to three housekeeping genes (HKG: HPRT, PPIA and GAPDH; △△Ct method). Differences relative to control cells are represented as boxplots with associated statistical significance (Mann Whitney; α = 0.05) along with Means ± SEM. *p < 0.05.