Figure 2 | Scientific Reports

Figure 2

From: Newcastle disease virus mediated apoptosis and migration inhibition of human oral cancer cells: A probable role of β-catenin and matrix metalloproteinase-7

Figure 2

NDV induced apoptosis in SAS cells. Images show analysis of apoptosis by NDV (0.1 MOI) on SAS by Annexin V FITC and PI assay using flow cytometry (A). Agarose gel image showing DNA laddering post-NDV infection in SAS cells (B). Hoechst 33342 staining of the cells showing apoptosis-related changes in the nucleus (C). Image showing the decreased mitochondrial membrane potential upon NDV infection (D). In mock-treated SAS cells, mitochondria aggregated JC-1 dye to give red fluorescence while NDV infected cells showed decreased fluorescence. Western blot analysis of cleaved PARP, cytochrome c, and caspase 3 upon NDV infection showed a band size of 89 kDa, 14 kDa and 17 kDa, respectively. β-actin was used as a loading control for the blots (E). “M” stands for the mock control, “L” for DNA ladder and STS for staurosporine.

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