Figure 3 | Scientific Reports

Figure 3

From: Newcastle disease virus mediated apoptosis and migration inhibition of human oral cancer cells: A probable role of β-catenin and matrix metalloproteinase-7

Figure 3

The images show NDV infection in SAS cells leading to migration inhibition. Wound healing assay was performed on SAS cells, the cells were infected with 0.1 MOI NDV and cell migration was evaluated at regular time points post-infection (A). The images were taken at 10X magnification under inverted microscope (Lebomed, USA). The open wound area was calculated at every time point by using ImageJ software and converted into a percentage with respect to zero hr (B). The mRNA levels of various MMP’s were analyzed 6, 12, 24 hr post-NDV infection, each experiment was repeated thrice using GAPDH as a normalizing control (C). Data is represented as fold change upon NDV infection relative to control. qRT-PCR was performed 3 times. T-test using Microsoft Excel, *P < 0.05, **P ≤ 0.01, ***P ≤ 0.001.

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