Figure 3
ER stress downregulated cytoplasmic and surface LAMP1. Immunocytochemical analysis showing staining of cytoplasmic LAMP1 (cLAMP1) in permeabilized HchEpC1b cells, or surface LAMP1 (sLAMP1) docked to the plasma membrane, in non-permeabilized HchEpC1b cells. (a) Representative panels showing the merged images of cLAMP1 (green) and nuclear staining (DAPI, blue) in HchEpC1b cells cultured with 500 ng/ml of brefeldin A (BFA) or 500 ng/ml of tunicamycin (TM) for 24 h. (b) The graph shows the intensity of cLAMP1 in HchEpC1b cells cultured with 500 ng/ml of BFA or 500 ng/ml of TM for 24 h. (c) Representative panels showing the merged images of sLAMP1 (green) and nuclear staining (DAPI, blue) in HchEpC1b cells cultured with 500 ng/ml of BFA or 500 ng/ml of TM for 24 h. (d) The graph shows the intensity of sLAMP1 in HchEpC1b cells cultured with 500 ng/ml of BFA or 500 ng/ml of TM for 24 h. (e) Acidic organelles, mainly lysosomes, are detected by LysoTracker staining. (f) The graph shows the number of LysoTracker-positive dots per cell in HchEpC1b cells cultured with 500 ng/ml of BFA or 500 ng/ml of TM for 24 h. Data expressed as mean ± S.D. *p < 0.05, **p < 0.01. Scale bars: 20 μm.
