Figure 5 | Scientific Reports

Figure 5

From: Endoplasmic reticulum stress disrupts lysosomal homeostasis and induces blockade of autophagic flux in human trophoblasts

Figure 5

Decrease in LAMP1 expression in serum of pre-eclampsia patients. (a) Western blot analysis showing LAMP1 and LAMP2 expression secreted in the medium of HchEpC1b cells cultured with DMSO (Cont), or 500 ng/ml of TM for 48 h, or culture medium without cells [Cell (−)]. (b) Western blot analysis showing LAMP1 and LAMP2 expression levels in human serum samples. The total protein levels on the membranes estimated by Ponceau S staining. The graph shows the expression levels of LAMP1 (c) or LAMP2 (d) in the sera. Expression is normalized to Ponceau S staining levels. (e) The graph shows the concentration of beta-galactosidase (b-gal) in human serum samples. Data expressed as the mean ± S.D. *p < 0.05, **p < 0.01, ***p < 0.001. NPS: normal pregnant sera, PES: pre-eclampsia sera. FGR: fetal growth restriction, Non-preg: Non-pregnancy.

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