Table 1 In vitro and in vivo development competence of cloned embryos of a superior buffalo bull.
Embryo type | Embryo cultured | In vitro development | In vivo development | |||||
---|---|---|---|---|---|---|---|---|
Cleaved n (%) | Blastocysts n (%) | Total cell number | Apoptotic index | Recipient animals | Pregnancy | Live birth | ||
SCNT skin-derived | 175 | 157 (89.0 ± 1.5) | 73 (40.4 ± 2.8) | 282.2 ± 38.08 | 8.5 ± 1.9a | 8 | 2 | 1 |
SCNT semen-derived | 203 | 185 (90.5 ± 3.5) | 70 (37.9 ± 8.5) | 310.6 ± 12.7 | 11.2 ± 1.8b | 7 | 0 | 0 |
IVF | 340 | 189 (55.5) | 52 (15.3) | 210.6 ± 45.6 | 6.36 ± 1.9a | — | — | — |