Table 1 In vitro and in vivo development competence of cloned embryos of a superior buffalo bull.

From: Successful cloning of a superior buffalo bull

Embryo type

Embryo cultured

In vitro development

In vivo development

Cleaved n (%)

Blastocysts n (%)

Total cell number

Apoptotic index

Recipient animals

Pregnancy

Live birth

SCNT skin-derived

175

157 (89.0 ± 1.5)

73 (40.4 ± 2.8)

282.2 ± 38.08

8.5 ± 1.9a

8

2

1

SCNT semen-derived

203

185 (90.5 ± 3.5)

70 (37.9 ± 8.5)

310.6 ± 12.7

11.2 ± 1.8b

7

0

0

IVF

340

189 (55.5)

52 (15.3)

210.6 ± 45.6

6.36 ± 1.9a

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  1. Data from 12 trails, values with different superscripts within the same column differ significantly (P < 0.05). In vitro fertilized (IVF) embryos produced using semen of donor bull as controls for cell apoptotic index analysis.