Figure 4

Evolution of fruit ripening control in the Solanaceae. (A) Expression (log₂ FPKM) of ripening transcription factor regulators CNR, NOR, RIN, TAGL1, FUL1/2, AP2a and ARF2A in different tissue types (root, leaf and flower) plus different stages of fruit ripening (stage 1, stage 2 and stage 3) of eggplant (upper panel), tomato (middle panel) and pepper (lower panel). (B) Co-expression (R-value ≥ 0.8) of ripening -related genes with the RIN ripening regulator; ellipses, diamonds and rectangles indicate, respectively, transcription factor-, receptor-, and enzyme-encoding genes; purple, red and green shading indicate, respectively, co-expression with RIN in eggplant, tomato and pepper. Detailed co-expression values are shown in Supplementary Table S31. (C) Wild-type tomato fruit at two stages of ripening (upper left two panels); wild-type eggplant fruit at two stages of ripening (upper right two panels); and over-expression of the TOMATO AGAMOUS-LIKE 1 (TAGL1) gene in tomato fruit at two ripening stages (lower left two panels) and in eggplant fruit at two ripening stages (lower right two panels).