Figure 1 | Scientific Reports

Figure 1

From: Rapid Detection of Genetic Engineering, Structural Variation, and Antimicrobial Resistance Markers in Bacterial Biothreat Pathogens by Nanopore Sequencing

Figure 1

Nanopore sequencing run metrics for Y. pestis strain S1037. Genomic DNA was extracted by the salt-precipitation (SP) or the silica-membrane (SM) method and libraries were prepared in triplicate using the ONT Rapid Sequencing Kit (Rapid), or the Field Sequencing Kit (Field). All run metrics were based on a 16 hour sequencing run time and the active pore count (-o-) was recorded during the platform QC step prior to the sequencing run. The number of raw fast5 reads generated (green, passed; yellow, failed) and raw FASTQ Albacore base-called reads with an average quality score (Q) of ≥ 7 (blue) are displayed.

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