Figure 3 | Scientific Reports

Figure 3

From: Rapid Detection of Genetic Engineering, Structural Variation, and Antimicrobial Resistance Markers in Bacterial Biothreat Pathogens by Nanopore Sequencing

Figure 3

Time required to detect antimicrobial resistance markers in Y. pestis and B. anthracis using nanopore whole genome sequencing. Time 0:00 starts from a pure isolated culture. The time to extract genomic DNA by the silica-membrane method is shown, including the one hour B. anthracis lysis step. Quality control steps include assessment of DNA concentration, quality, and integrity. Library preparation time is estimated for the Rapid Sequencing Kit. The average time required to generate 100,000 passed fast5 reads is shown for each species. Genome analysis includes base-calling using Albacore and de novo genome and plasmid assembly. See methods and Supplementary Figure 9 for the bioinformatics analysis pipeline details.

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