Figure 4

Detection of multimeric forms of pPCP1 in nanopore sequence assemblies of Y. pestis S1037. pPCP1 is ~9.5 kb, but the pPCP1 1-mer assembly is ~9.1 kb due to the presence of small indels in the nanopore-generated sequence. Reads >18.2 kb were assigned to the 3- or 4-mer form based on length. A representative read (grey) for each multimer was selected as a reference for the complete plasmid the remaining reads were aligned against this sequence (purple). Coding sequences (green) and IS100 elements (yellow) are shown in the center. The 2-, 3- and 4-mer forms are linked together in a 5′-3′ head-to-toe orientation.