Figure 5
Pharmacological profile of PhENAQ and effect on rhythmic LFPs in blind retinae. (a) Raster plot and histogram of representative MEA recording in an rd1/rd1,Opn4−/− retina after treatment with 200 µM PhENAQ. (b) Same as in a, with additional application of DNQX and D-AP5. (c) Same as in a, with additional application of CdCl2. (d) Quantification of light-on responses using the photoswitch index. (e) Graphical overview of pharmacological experiments. (f) Power spectrum of local field potentials before and after application of PhENAQ. (g) Quantification of maximal frequency response before and after application of PhENAQ (average intensities of complete time range). Maximum frequency with significant power is defined by the peak of the highest frequency reaching a threshold of 4 SD. Each data point refers to the power average of local field potentials in one retina (n = 7 retinas). The average change in power is depicted in red with error bars (mean ± SEM). (h) Quantification of maximum power at frequency peaks around 5 (black) and 10 Hz (blue) before and after application of PhENAQ (empty and filled circles, respectively).
