Figure 2
Increased of hsa-miR-4756-3p induced apoptosis, cell cycle arrest and inhibit cell proliferation, migration in vitro. Control miRNA and hsa-miR-4756-3p mimic was transfected in TNBC cell line MDA-MB-231 cells for 48 h, then using (A)Annexin V APC/7-AAD double staining to detect the change of cell apoptosis. (B) CCK8 to detect cell proliferation change. (C) Wound healing assay to assess cell migration change. (D) High concentration PI staining to assess cell cycle change. (E) MDA-MB-231 cell was transfected with hsa-miR-4756-3p mimic, then TGFβ-1 pathway molecules TGFβ-1, TGFβ-1 type 1 receptor, TGFβ-1 type 2 receptor, SMAD3, p-SMAD3 were detected by western blot. (F) MDA-MB-231 cell were injected in mammary gland fat pad of nude mice, then control miRNA and hsa-miR-4756-3p inhibitor were inject in nude mice using DOPC liposomes, after 1 months, mice were sacrificed, and primary tumor diameter were assessed.
