Figure 5
Standard curves for several protein quantification methods. Standard curves of protein amount via (A) TCE, (B) A280, and (C) Bradford assays. (A) Fluorescence intensity (FI; emission ʎ = 450 nm; excitation ʎ = 310 nm) of 0–200 µg BSA following 15 minutes of UV-exposure in the presence of 0.5% (v/v) TCE. (B) Absorbance measurements (ʎ = 280 nm) of 0–200 µg BSA. (C) Absorbance measurements (ʎ = 595 nm) of 0–40 µg BSA in Bradford reagent. Error bars represent propagated standard deviations of blank corrected means. The interpolated standard curve and 95% confidence intervals are represented by solid and dotted blue lines, respectively. The limit of detection (LOD) is depicted by the red dotted lines.
