Figure 1
From: Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology
Schematic of the Replication Protocol for Replica Leaf Surfaces in PDMS. (a) Affix a living leaf sample to a petri dish with double-sided tape. (b) PDMS with a ratio of 20:1 is then poured on to the affixed leaf, with the PDMS degassed and cured. (c) The leaf imprint is then carefully peeled off. Prior to pouring the leaf imprint with PDMS for double-casting, an anti-adhesion coating is applied. (d) PDMS with a ratio of 10:1 is poured onto the imprint, with the PDMS being degassed and cured. (e) Once cured the leaf replica is peeled carefully from the leaf imprint.
