Figure 5
From: Structural analysis of biological targets by host:guest crystal lattice engineering
L2 construct with DARPin_3G124. (A) Crystals of the EngBF-L1-DARPin_B6:c-pep1 complex (top) and EngBF-L2-DARPin_3G124 co-crystallised with sfGFP (bottom). (B) Structure of EngBF-L2-DARPin_3G124 (light blue for EngBF part and yellow cartoon for fused DARPin). Inserted cysteines are depicted as spheres and the symmetry-related molecule is in grey. (C) Final 2mFo − DFc map of the DARPin_3G124 ___domain. The map was contoured at 1.0σ. (D) DARPin_3G124nc:sfGFP complex (PDB-ID 5MA6, grey Cα-trace) superimposed on EngBF-L2-DARPin_3G124 (orange cartoon). The mFo − DFc map was contoured at +3.1σ (green) and −3.1σ (red) and is shown around the sfGFP with an 8 Å cushion. (E) B-factor colouring of EngBF-L2-DARPin_3G124 (surface) with values ranging from 31 Å2 (blue) to 252 Å2 (red). Symmetry mates are shown as grey surfaces. The superimposed sfGFP is shown as a grey cartoon to indicate the position of the target.
