Figure 2
From: Identification and Validation of a Novel Biologics Target in Triple Negative Breast Cancer
GABRP protein expression in breast cancer cell lines. (A) GABRP protein was detected in Western blots using intracellular ___domain (ICD) and extracellular ___domain (ECD) binding GABRP antibodies. LRP8, another potential target overexpressed in TNBC, was also assessed. HER2 served as a control for HER2 amplified cell lines (SKBR3 and BT-474), while actin was used as a loading control. (B) Membrane and cytoplasmic protein extracts were obtained by subcellular protein fractionation. Western blot analysis was performed to determine GABRP levels using these subcellular protein fractions of each cell line. Na+K+ATPase was used as a positive control for membrane protein, while actin was used as a loading control.
