Figure 2
From: A micropatterned substrate for on-surface enzymatic labelling of linearized long DNA molecules
Combing λ-DNA on micropatterned, dual-functionalized glass. λ-DNA molecules were combed onto 10–15 (A) and 10–40 (B) substrates. Octenyl sections appear as bright strips due to adsorption of YOYO-1 dye in contrast to the low-fluorescence background of PEG sections. Molecules were predominantly end-bound to the octenyl sections and extended across the PEG sections. Both, 10–15 and 10–40 resulted in similar linearization, with relatively lower DNA density on the 10–40. (C) Overlaid histograms with matching Gaussian regressions reveal the length distributions of λ-DNA molecules combed on unpatterned octenyl substrate (blue, n = 191) and micropatterned octenyl-PEG (red, n = 116). octenyl-PEG substrate produced significantly lower DNA extension.
