Figure 4
From: A micropatterned substrate for on-surface enzymatic labelling of linearized long DNA molecules
Characterization of low-fluorescence background; on-surface nick-labelling of hgDNA; on-surface transcription on T7 DNA. (A) A magnified image of an octenyl section and adjoining PEG sections (10–40) shows suppressed binding of ATTO-532-dUTP (150 nM) to PEG compared to octenyl. (B) ATTO-532-dUTP was successfully incorporated into combed hgDNA molecules using nick-labelling chemistry. (C) Transcription reaction performed using T7 RNAP on combed T7 DNA resulted in bright, labelled RNA aggregates detected along the T7 backbone.
