Figure 5
From: A micropatterned substrate for on-surface enzymatic labelling of linearized long DNA molecules
On-surface optical mapping of λ-DNA. (A) top: BbvCI site (red dots) distribution on λ-DNA (blue); bottom: simulated appearance of the BbvCI-map of λ-DNA (B,C) Microscope images of on-surface nick-labelled λ-DNA molecules that were concatemerized, combed, labelled, and stained. The green arrows point to λ-DNA molecules that contain the 4 BbvCI nick-labels and the blue arrows point to partially and/or weakly labelled molecules. (D) A collection of nick-labelled λ-DNA molecules aligned against the reference BbvCI map for λ-DNA (E) Histogram showing the predicted BbvCI nick-label positions on λ-DNA backbone. The predicted positions were found to be 12.0, 17.1, 29.6, and 39.6 kbp corresponding to the simulated label positions, 12.7, 17.1, 30.2, and 40.5 kbp respectively.
