Figure 4 | Scientific Reports

Figure 4

From: Distinct downstream signaling and the roles of VEGF and PlGF in high glucose-mediated injuries of human retinal endothelial cells in culture

Figure 4

The abundances of PlGF and VEGF are increased in HG-treated hRECs. hRECs were treated with high glucose (HG, 25 mM) for the indicated time periods. Non-treated cell (CTL) or mannitol (MN) treated cell was used as the controls. (a). Quantitative real PCR was performed to assess the mRNA levels of VEGF and PlGF. (b). Culture medium was collected and ELISA was performed to quantitate the amount of secreted PlGf and VEGF, respectively. (c). Cells were lysed and total protein was extracted for immunoblotting with anti-PlGF and anti-VEGF antibodies. Full length blots are shown for PlGF and VEGF. MN-treated hRECs were used as the treatment control (Supplementary Fig. 9). Data are presented mean ± SD. n = 3. b: *p < 0.05, **p < 0.01, ***p < 0.001. n.s. non-significance. a and c: *p < 0.01 vs 0 h.

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