Figure 6 | Scientific Reports

Figure 6

From: The Interactome analysis of the Respiratory Syncytial Virus protein M2-1 suggests a new role in viral mRNA metabolism post-transcription

Figure 6

Interaction between M2-1 and various fragments of PABPC1. (a) The NanoLuc Two-Hybrid assay was used to detect the interaction between M2-1 and 5 fragments of PABPC1. Cells were treated with RNAse A 1 h prior to luminescence measurement. Results are displayed as a normalized luminescence ratio (NLR) of the signal of the two fusion proteins over two control protein pairs. The data was collected on 4 experiments performed in duplicates. (b) A schematic representation of PABPC1’s domains, with numbers representing amino acid positions of domains boundaries45. Below are represented the fragments of PABPC1 tested for interaction with M2-1. The numbers correspond to the positions of the first and last amino acid of each fragment.

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