Figure 2
PPG neurons express glutamic acid decarboxylase 67 but not 65. (a) Representative photomicrograph of the GCL in PPG-Cre-RFP transgenic mice (red channel; left) whereby glutamic acid decarboxylases (GAD) GAD65 and (b) GAD67 are visualized using immunocytochemistry (green channel; middle). (c) Representative photomicrograph of the GCL in progeny resulting from a cross between PPG-Cre-RFP x GAD67-GFP mice. Arrow = PPG neurons that co-express RFP and GAD67. PPG neurons largely did not express GAD65 as evidenced by a lack of RFP and GAD65 co-labeled neurons. (d) Representative photomicrograph of the GCL in PPG-Cre-RFP transgenic mice (red channel; left) where the vesicular GABA transporter (VGAT) is visualized by immunohistochemistry (green channel; middle).
