Figure 6
Light-stimulation of PPG neurons induces a biphasic inhibition-excitation response in mitral cells. (a) Schematic representation as in Fig. 2 with electrode placement on the M/TC, and optical stimulation of the GCL (blue ellipse). (b) (top, left) Twenty current-clamp traces recorded from a M/TC showing a biphasic inhibition-excitation response after light-stimulation (tick) of PPG neurons, (dashed inset) altered time scale resolution of the light-stimulation event (right). (bottom, left) Peristimulus time histogram (PSTH) of all events (20 ms bins). (c) Different example of a silent M/TC where light-stimulation induces an inhibitory post-synaptic potential (IPSP) only. (top) Recordings of a single M/TC under ACSF conditions (Control), (middle) following bath application of GBZ, and (bottom) and then following bath application of APV, and NBQX. (d) Current-clamp recordings from a M/TC where light-induced IPSPs are observed using potassium-methane-sulfonate (KMeSO3) as the internal solution. (top) Using ACSF conditions (Control) and following (bottom left) bath application of GBZ or (bottom right) APV + NBQX.
