Table 1 Intrinsic membrane properties and light-elicited currents in olfactory bulb neurons.
Cell type | PPG-neuron | Granule Cell | M/T Cell | |||
|---|---|---|---|---|---|---|
Membrane properties | ||||||
Input resistance (MΩ) (n) | 850 ± 154 (15) | 748 ± 330 (7) | 142 ± 5 (195) |  |  |  |
Capacitance (pF) | 15.71 ± 0.63 | 10.35 ± 1.52 | 90.25 ± 1.71 |  |  |  |
Resting potential (mV) | –75.07 ± 1.63 | N/A | –64.39 ± 0.59 |  |  |  |
Spontaneous action potentials | None | Yes | Variable | Â | Â | Â |
Light-induced current properties # | ||||||
Current type | ChR2 | EPSC | IPSC | EPSC | IPSC | EPSC |
Vhold | −70 mV + 1 µM TTX | −70 mV |  + 0 mV | −70 mV |  + 0 mV | −70 mV |
Intracellular solution (n) | KGluc (8) | CsMeSO3 (6) | CsMeSO3 (9) | CsMeSO3 (9) | Â KGluc (15) | Â KGluc (15) |
Latency (ms) | 0.13 ± 0.04 | 12.50 ± 1.60 | 11.17 ± 0.58 | 6.69 ± 1.09*** | 8.59 ± 0.74 | 3.18 ± 0.29*** |
Amplitude (pA) | 118.46 ± 29.24 | 78.04 ± 5.78 | 245.00 ± 44.64 | 87.38 ± 16.46*** | 38.33 ± 5.28 | 17.75 ± 2.36*** |
Rise time (ms) | 2.89 ± 0.91 | 2.61 ± 0.65 | 6.73 ± 0.66 | 9.36 ± 3.74 | 5.23 ± 1.34 | 12.74 ± 2.21*** |
Rise slope (pA/ms) | 52.16 ± 23.25 | 25.75 ± 4.31 | 25.37 ± 6.08 | 10.86 ± 3.28*** | 7.41 ± 1.79 | 1.24 ± 0.34*** |
Decay time (ms) | 18.27 ± 1.36 | 15.31 ± 12.32 | 39.38 ± 3.84 | 102.62 ± 79.48* | 15.07 ± 2.41 | 51.59 ± 6.49*** |
Decay slope (pA/ms) | 3.46 ± 0.87 | 5.82 ± 1.48 | 3.69 ± 0.71 | 2.19 ± 0.64** | 1.85 ± 0.53 | 0.23 ± 0.05*** |
½ width (ms) | 12.62 ± 0.37 | 5.27 ± 2.63 | 31.83 ± 1.26 | 30.28 ± 8.85 | 15.34 ± 2.01 | 39.82 ± 5.76*** |
Area (pA.ms) | 2710 ± 909 | 1186 ± 247 | 10200 ± 2093 | 3427 ± 1125*** | 763 ± 143 | 1045 ± 206*** |
Synaptic blockers sensitivity | None | APV  + NBQX | GBZ and APV  + NBQX | APV  + NBQX | GBZ and APV + NBQX | APV   + NBQX |
