Figure 4
No significant change of HIF-1α expression in macrophages in CLS. (A) HIF-1α staining in SAT and VAT. HIF-1α can be detected in both cytoplasm and nucleus (red arrows). HIF-1α stained in both cytoplasm and nucleus of macrophages in WT mice (a,b). In ERβ−/− mice, HIF-1α also stained in both cytoplasm and nucleus of macrophages (c,d). Macrophages outside CLS were not hypoxic in WT mice (black arrows in insert pictures in b). (B,C) No significant change in ratio of nuclear HIF-1α-positive cells was found between WT mice and ERβ−/− mice. (Scale bars in Aa–d, 50 μm).
