Figure 2
trpc1 is expressed in the ectoderm of blastula and gastrula stage embryos. Spatial expression of trpc1 mRNA in blastula and gastrula stage embryos. Whole mount in situ hybridization was performed on embryos fixed at blastula (stage 9), early gastrula (stage 10.5) and late gastrula (stage 11.5). (A) Expression of trpc1 at stage 9 in an intact embryo (animal pole view), showing that the ectoderm is labelled. (B) Sagittal section taken through the embryo shown in panel A, indicating trpc1 expression in the ectoderm alone; the mesoderm and endoderm were not labelled. (C) Expression of trpc1 in an intact embryo at stage 10.5 (animal pole view). (D) Sagittal section taken through the embryo shown in panel (C), showing trpc1 expression in the ectoderm, with no difference in the level of expression between the dorsal and the ventral sides. (E) Expression of trpc1 in an intact embryo at stage 11.5 (animal pole view). (F) Sagittal section taken through the embryo shown in panel E. AP, VP, ect, mes, end and Bl are animal pole, vegetal pole, ectoderm, mesoderm endoderm and blastocoel, respectively. In panels (D) and (F), dorsal is to the right and the arrows indicate the blastopore lip. Scale bar is 300 µm.
