Figure 5
Antibody immune responses to ChAdOx1 MERS in dromedary calves at different ages. Seronegative dromedary calves, below 1 year old, were immunised with PBS (n = 2) or ChAdOx1-eGFP (n = 1) in group AG01 (black triangles) or with ChAdOx1 MERS in group AG02 (n = 3; blue squares). Older calves, 1–2 years old, were immunised with ChAdOx1 MERS in group AG03 (n = 3; red circles). (A) Anti-spike Ab titres were assessed in ELISA; and nAb titres were determined in MERS-CoV neutralisation assay (B) and in MERS pseudotyped virus neutralisation assay (MERSpp NA) (C). (D) Linear regression between the values of MERS-CoV neutralisation assay and MERSpp NA showed a significant relationship with R2 = 0.96, p < 0.0001. (A) Nasal mucosal nAbs were determined by MERSpp NA in samples from 0 and 21 d.p.c.
