Figure 1

The AXL mRNA expression is correlated with AXL 5′-UTR reporter activity. (A) Inverse correlation between AXL mRNA level and AXL promoter reporter activity. CL1-0, CL1-3 and CL1-5 cells were transfected with the pGL3-AXL promoter luciferase construct, respectively. Luciferase reporter activity was assayed 48 h later. (B) The steady-state AXL mRNA levels of cell lines were compared by RT-PCR using GAPDH as an internal control. (C) AXL 5′-UTR reporter activity in CL1-0 and CL1-5 cells. The pMIR-Report-AXL 5′-UTR or p-MIR-Report (mock vector) plasmid was co-transfected with Renilla plasmid (serving as an internal control for transfection efficiency) into CL1-0 and CL1-5 cells. After 24 h, luciferase activity of the cell lysates was measured. Relative fold change in luciferase activity was plotted with respect to the pMIR-Report mock control. All reporter assays were performed in triplicate and the Renilla reporter activity was used to normalize the transfection efficiency. The error bars represent SD. *P < 0.05.