Figure 7 | Scientific Reports

Figure 7

From: Aspirin enhances cisplatin sensitivity of resistant non-small cell lung carcinoma stem-like cells by targeting mTOR-Akt axis to repress migration

Figure 7

Cisplatin impedes migration by deterring Integrin/Fak signalling in aspirin pre-treated spheroids. (A) Percent Integrin-α2 (left panel), Integrin-α5 (middle panel) and Integrin-β1 (right panel) expression was represented graphically as detected by flow-cytometry in A549 parental cell and spheroids. (B) Western blot analysis of Integrin-α2, Integrin-α5, Integrin-β1 and p-FAK in untreated or aspirin/-cisplatin/-aspirin + cisplatin treated A549 spheroids. α-Actin was used as loading control. (C) Percent cell migration was calculated and represented graphically in control and Integrin-α2-siRNA, Integrin-α5–siRNA or Integrin-β1-siRNA transfected A549 spheroids as detected by transwell migration assay. The inset shows the immunoblot analysis of Integrinα2, Integrinα5 or Integrinβ1 levels for the transfection efficiency of Integrin-α2-siRNA, Integrin-α5–siRNA and Integrin-β1-siRNA respectively. (D) Protein expression levels of MMP-2 and MMP-9 in untreated or aspirin + cisplatin treated A549 spheroid. α-Actin served as loading control. Values are mean ± SEM of three independent experiments in each case or representative of typical experiment *p < 0.05, **p < 0.01, ***p < 0.001.

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