Figure 1

Aged Plcg1^F/F; Dlx5/6-Cre mice exhibit handling-induced seizures and behavioral aberrations. (a) Experimental design for the generation of Plcg1^F/F; Dlx5/6-Cre mice. (b) Normal behavior in control mice and handling-induced seizures in Plcg1^F/F; Dlx5/6-Cre mice. (c) Percentage of seizure occurrence by the genotypes (control, 2 of 13 mice; Plcg1^F/F; Dlx5/6-Cre, 12 of 16 mice; Chi-square test, **P = 0.0014). (d) The number of mice at age of seizure onset. (e) Assessment of seizure grade in Plcg1^F/F; Dlx5/6-Cre mice by Racine scale. (f) Representative tracing of open-field test for control (n = 9) and Plcg1^F/F; Dlx5/6-Cre mice (n = 11). (g) Distance travelled with 10 min interval in the open-field test (two-way repeated measures ANOVA, genotype effect, *P = 0.0105). (h) Total distance travelled in the open-field chamber (unpaired t-test, **P = 0.0077). (i) Time spent moving in open-field chamber (unpaired t-test, **P = 0.0012). (j) Thigmotaxis in the open-field test (two-way repeated measures ANOVA, genotype effect, **P = 0.0077, **P_{periphery =}0.0046 in Sidak’s multiple comparison test). (k) Representative tracing of elevated plus maze for control (n = 12) and Plcg1^F/F; Dlx5/6-Cre mice (n = 9). (l,m) Time spent in open (l) and closed arm (m) of elevated plus maze (unpaired t-test, ***P_open = 0.0002, ***P_closed = 0.0004). (n,o) Entry number in open (n) and closed arm (o) of elevated plus maze (unpaired t-test, **P_open = 0.0034, *P_closed = 0.0158). (p) Experimental scheme for contextual fear conditioning and memory. (q) Contextual fear memory 24 hours after conditioning (control, n = 8; Plcg1^F/F; Dlx5/6-Cre, n = 11; unpaired t-test, *P = 0.0433). (r) Percentage of spontaneous alteration for 10 min in the Y-maze test (control, n = 8; Plcg1^F/F; Dlx5/6-Cre, n = 11; unpaired t-test, P = 0.4975). (s) Immobility time in the tail suspension test (control, n = 8; Plcg1^F/F; Dlx5/6-Cre, n = 11; unpaired t-test, P = 0.8725). (t) Immobility time in the forced swim test (control, n = 8; Plcg1^F/F; Dlx5/6-Cre, n = 11; unpaired t-test, P = 0.7039). All data were expressed as mean ± SEM.