Figure 3
From: Nano-encapsulated Escherichia coli Divisome Anchor ZipA, and in Complex with FtsZ
Interaction of SMALP-ZipA with FtsZ as monomers and polymers. (a) SvAUC analysis of SMALP-ZipA (blue), FtsZ (red) and combination of FtsZ and ZipA in a 1:1 ratio (green). (b) SDS-PAGE Coomassie blue-stained gel for the Supernatant (S) and pellet (P) of FtsZ (11 μM) and SMALP-ZipA (3.3 μM) (Lanes 5 and 6), with negative control, ZipA (3.3 μM Lanes 1 and 2) and positive control, FtsZ (11 μM Lanes 3 and 4) all in polymerisation buffer (50 mM MES pH 6.5, 50 mM KCl, 2.5 mM MgCl2 and 2 mM GTP. (c) TEM negative stain micrographs show the effect of increasing amounts of SMALP-ZipA on FtsZ (5.5 µM) in polymerisation buffer. SMALP-ZipA concentrations (i) 0 µM, (ii) 0.375 µM, (iii) 0.75 µM, (iv) 1.15 µM, (v)1.5, (vi) expanded 0.75 µM. White arrows point to the fibre and blue arrow indicates SMALP-ZipA.
