Figure 5
From: Nano-encapsulated Escherichia coli Divisome Anchor ZipA, and in Complex with FtsZ
SAXS and SANS2D analysis of SMALP-ZipA. (a) Experimental SAXS curves of SMALP-ZipA. A straight-line in the Guinier plot is indicative of Guinier scaling with Rg of 4.2 nm. (b) Pair distribution function P(r) of SMALP-Zip shows a Dmax of 12 nm. (c) Dummy atom model of the SAXS reconstructed envelop from DAMMIF, showing orthologous views. The dimensions show a length og 16.5 nm, width 10 nm, and 6.5 nm for side-on or face-on view respectively. Front and side views are of docking SMALP-ZipA Cryo-EM structure file (.pdb file) into the DAMMIF envelope are displayed. (d) SANS2D data collected of SMALP-ZipA in four different contrasts of H2O/D2O, shows different features in each data point (e) DAMMIF models of SANS2D data at four different contrasts. (f) Orthogonal views of surface contour of SMALP-ZipA DAMMIF SANS2D model with the X-ray crystal structure of the GD of ZipA (1F47).
