Figure 6
Inhibition of AT1R and AT2R diminishes Ang II-induced necroptotic HK-2 cells. (A) TUNEL-stained (green fluorescence) HK-2 cells treated with 10−9 M Ang II for 24 h were costained to detect RIP3 (red fluorescence) and the nuclei (DAPI, blue fluorescence). The scale bars represent 50 μm. (B) Representative TEM images of necroptotic HK-2 cells (as shown by the arrow) after treatment with 10−9 M Ang II for 24 h. The scale bars represent 5 μm. (C) The data are presented as the % ratio of necroptotic HK-2 cells(as shown by the arrow). (D) The data are presented as the % ratio of necrotic cells among all HK-2 cells treated with 10−9 M Ang II for 24 h. For the data in (C, D), the values are reported as the means ± S.E.M. of n = 6; **p < 0.01, versus control; ##p < 0.01, versus the Ang II-treated group. Ang II: angiotensin II.
