Figure 3

Selected reaction monitoring (SRM) chromatograms of mixture of 18 bile acid standards. The standard mixture was labeled with either 2PA or 2PA-d6, and 1:1 mixture of the labeled compounds was analyzed by RPLC triple-quadrupole mass spectrometry. LCA: lithocholic acid, UDCA: ursodeoxycholic acid, HDCA: hyodeoxycholic acid, CDCA: chenodeoxycholic acid, DCA: deoxycholic acid, wMCA: ω-muricholic acid, aMCA: α-muricholic acid, bMCA: β-muricholic acid, HCA: hyocholic acid, CA: cholic acid, GLCA: glycolithocholic acid, GUDCA: glycoursodeoxycholic acid, GHDCA: glycohyodeoxycholic acid, GCDCA: glycochenodeoxycholic acid, GDCA: glycodeoxycholic acid, GaMCA: glyco-α-muricholic acid, GbMCA: glyco-β-muricholic acid, GHCA: glycohyocholic acid, GCA: glycocholic acid.