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Figure 1

From: Quantitative Phase Imaging Using Digital Holographic Microscopy Reliably Assesses Morphology and Reflects Elastic Properties of Fibrotic Intestinal Tissue

Figure 1

Experimental set-up. (A) Full thickness surgical resection specimen of Crohn’s disease patients with a stricturing disease phenotype were obtained from the stenotic segment and the adjacent, non-stenotic segment of the intestinal wall. (B) Experimental setup for off-axis digital holographic microscopy (DHM) and bright field imaging; (C) Bright field image of representative stenotic tissue; (D) corresponding digital off-axis hologram; (E) quantitative phase image reconstructed from the digital hologram in D; (F) enlarged part of the digital hologram that illustrates the off-axis carrier fringes; (G) false color coded pseudo 3D representation of the quantitative phase image in E.

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