Figure 1
Pathogenicity in mice. Five mice per group were intranasally inoculated with 106.0 EID50 of PBS, WT or MA. (A) Mouse body weights were monitored daily for 14 days. The values are the average scores of the overall body weight loss with respect to the initial body weights, ±standard deviations (SDs). (B) The survival percentages were calculated by observing the infected mice. (C) Lungs were collected from mice inoculated with 106.0 EID50 WT or MA at 1, 3, 5 and 7 dpi (n = 3), virus titers were determined in 9-day-old SPF embryonated eggs (EID50/gram). Briefly, the lung tissues were weighed, and 0.1 grams of each tissue was placed into 1 ml of PBS containing 100 U/ml penicillin, to make 10% weight/volume lung homogenates (*P < 0.05; **P < 0.01).
