Table 1 Results from four replicates of influenza viral neuraminidase at various concentrations using the master mix formulation.

From: A Biochemiluminescent Sialidase Assay for Diagnosis of Bacterial Vaginosis

Input enzyme (pg/reaction)

Mean RLU (n = 4)

% CV

S/N

250,000

4,314,512 ± 98,412

2.28

569.90

125,000

3,725,267 ± 227,959

6.12

492.07

62,500

2,860,471 ± 178,124

6.23

377.84

31,250

1,821,260 ± 38,316

2.10

240.57

15,625

1,068,100 ± 88,771

8.31

141.09

7,800

633,784 ± 53,695

8.47

83.72

3,900

346,125 ± 31,569

9.12

45.72

1,950

182,087 ± 17,462

9.59

24.05

1,000

104,170 ± 7,486

7.19

13.76

500

76,776 ± 2,373

3.09

10.14

100

23,088 ± 1,308

5.66

3.05

50

17,051 ± 1,093

6.41

2.25

10

10,499 ± 1,108

10.56

1.39

0

7,571 ± 183

2.42

1.00

  1. The signal to noise (S/N) ratio was calculated by dividing the signal from reactions containing the input enzyme with the background signal of the reaction without input enzyme. RLU: relative light units. CV: coefficient of variation.
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