Figure 4

UV-B irradiation does not impair the differentiation capacity of MSCs. (a) BODIPY (493/503) staining of lipid droplet showing adipogenic differentiation of MSCs after exposure to UV-B light (scale bar 1000 µm). (b) OsteoImage™ staining for quantification of hydroxyapatite formation in differentiated MSCs after UV-B treatment (scale bar 1000 µm). (c) Aggrecan staining demonstrating chondrogenic differentiation in untreated and UV-B-exposed MSCs (scale bar 100 µm). *P < 0.05, **P < 0.01, ***P < 0.001. Bars show mean values, while error bars represent standard deviation, n ≥ 4.