Figure 5
From: Human mesenchymal stem cells are resistant to UV-B irradiation
MSCs exhibited low apoptosis levels after UV-B irradiation. (a) Percentage of apoptotic cells assessed by sub-G1 population and caspase-3 activation in MSCs and HS68 at 24, 48 and 96 hours after irradiation with UV-B light. Data are shown as mean ± standard deviation, n = 3. (b) Relative β-galactosidase staining intensity after 25 mJ/cm2 or 100 mJ/cm2 UV-B irradiation. Representative images show β-galactosidase expression as cellular senescence marker 96 hours after UV-B treatment (scale bar 200 µm). *P < 0.05, **P < 0.01, ***P < 0.001. Data are mean ± standard deviation, n = 4.
