Figure 7
From: Extracellular NAD+ enhances PARP-dependent DNA repair capacity independently of CD73 activity
Impact of NAD+ depletion and NAD+ precursor supplementation on DNA repair complex formation. (A, B) Comparative measurements of maximal recruitment of XRCC1-mCherry protein to the site of laser-induced DNA damage: MCF-7/Cas9 vs MCF-7/CD73-KO cells exposed to NAD+ in the presence of serum-free media (SFM) or media supplemented with fetal bovine serum (FBS) or heat inactivated fetal bovine serum (FBS-HI) for 6 hrs (A) or 24 hrs (B). (C) Representative images of maximal recruitment of XRCC1-mCherry in MCF-7/Cas9 cells cultured in media+FBS, SFM or media+FBS-HI; respectively, with differing NAD+ status.
