Figure 3

Phylogenetic analysis based on the nucleotide sequence of the MRV-2 Osaka strains. (A) Maximum-likelihood phylogram based on partial nucleotide sequences of the S1 segment (MRV-2 Osaka strains, 1,023 bp). The MRV-2 branch is enlarged in (B). (C) Maximum-likelihood phylogram based on partial nucleotide sequences of the S2 segment (MRV-2 Osaka strains, 1,155 bp). Solid squares indicate the strains isolated in this study. Each bracket indicates a cluster that includes the strain isolated in this study. Each strain ID consisting of MRV type, accession number, host species, three-letter country name abbreviation, and detection year is based on data obtained from GenBank in February 2019. The names of MRV prototype strains and other important strains to this study are enclosed in double quotes and presented at the end of each strain ID. On the basis of Bayesian information criteria, a general time reversible plus gamma plus invariable sites model was used to construct the phylogram. Numbers at the nodes indicate the bootstrap support values, which are given as a percentage of 1,000 replicates (values less than 80% are omitted). Scale bar indicates genetic distances (nucleotide substitutions per site). Country abbreviations include: AUT, Austria; CAN, Canada; CHE, Switzerland; CHN, China; CMR, Cameroon; DEU, Germany; FRA, France; HUN, Hungary; ITA, Italy; JPN, Japan; KOR, South Korea; NLD, The Netherlands; RUS, Russia; SVN, Slovenia; TWN, Taiwan; and USA, United States of America.