Figure 3

GSH production from bacterial lysates. (A) Bacterial lysates derived from C. rodentium strains grown in the absence of H2O2 were incubated with 1 mM CDNB and 1 µM GST. Absorbance was recorded at 340 nm every 20 seconds for 10 minutes and OD340 data were converted into GSH concentrations using a GST standard curve. (B) GSH production from C. rodentium strains grown in the presence of H2O2. (C) Complementation of C. rodentium nleB growth in 2.4 mM H2O2 in the presence or absence of 5.0 mM GSH.