Figure 8

Localization of FRMD6-immunoreactivity in L4-5 spinal cord one week after sciatic nerve axotomy. (A,B) FRMD6-immunoreactivity is increased in the ipsilateral compared to the contralateral dorsal horn (DH). (C) Mean intensity of FRMD6-immunoreactivity in the superficial layers (outlined by yellow dashed lines) is significantly increased on the ipsilateral side (n = 3 spinal cord). (D,E) Triple labeling for FRMD6, CGRP and Hoechst in the contralateral (D) and ipsilateral DH (E). Note increase in FRMD6-immunoreactivity and decrease in CGRP-immunoreactivity. (F–K) High magnification micrographs show FRMD6-immunoreactivity is increased in ipsilateral (I,K) than contralateral side (F,H). (L–O) After triple staining (O) for FRMD6 (L), CGRP (M) and Hoechst (N), FRMD6-immunoreactivity is seen in a cell body (arrowheads) and processes (arrows) of a CGRP⁺ motor neuron in the ipsilateral ventral horn (VH). Weak nuclear labeling for FRMD6 is seen in CGRP⁺ (left double arrowheads) or CGRP-negative (right top double arrowheads) neurons. Scale bars indicate 100 μm (A,B), 50 μm (D,E) and 10 μm (F–O).