Figure 3
From: Structural basis for Glycan-receptor binding by mumps virus hemagglutinin-neuraminidase
STD NMR analysis of reducing Sia produced during the hydrolysis of 1 by MuV-HN. (a) The off-resonance spectrum as reference (black) and the STD (red) of MuV-HN–trisaccharide-1 mixture upon hydrolysis reaction. (b) Epitope map of reducing Sia. STD percentages were calculated by (I0 – Isat)/ I0 ratio, normalized with respect to the highest STD signal of the acetyl group belonging to sialic acid. Chem Draw 2006 Software (http://www.cambridgesoft.com) was used to draw the sugar. The NMR spectra were plotted by using the xwinplot tool of TopSpin 3.6.1 software (www.bruker.com).
