Figure 4 | Scientific Reports

Figure 4

From: Human cytomegalovirus promoting endothelial cell proliferation by targeting regulator of G-protein signaling 5 hypermethylation and downregulation

Figure 4

Interaction of HCMV and environmental risk factors on endothelial cell proliferation by DNA methylation. (A) Changes in cell proliferation function in each treatment group by CCK-8 analysis. (B) Detection of methyltransferase activity in different treatment groups. (C) The methylation levels of CpG sites in RGS5 promoter regions from the four groups. (D) Effect of DAC on the expression of RGS5mRNA.*P < 0.05 vs control; #P < 0.05 HCMV+HG + ox-LDL vs HCMV or HG + ox-LDL. (E) Effect of DAC on cell proliferation. (F,G) Effect of DAC on the expression of PCNA protein. Data are expressed as means ± standard deviations. *P < 0.05 vs control; #P < 0.05 HCMV + HG + ox-LDL vs HCMV or HG + ox-LDL. ΔP < 0.05 HCMV vs HCMV + DAC, HG + ox-LDL vs HG + ox−LDL + DAC. Student’s t-test.

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