Figure 3

TJ barrier in lesional AD skin and its correlation to Cldn-1 levels. (a,a′,a′′) Example for Biotin-556-TJ barrier assay in lesional AD skin. (b,b′,b′′) Double-staining of Biotin-556 (red) and occludin (green), (c,c′,c′′) of Biotin-556 (red) and Cldn-1 (green), and (d,d′,d′′) of Biotin-556 (red) and Cldn-4 (green) in lesional AD skin. (a′,b′,c′,d′) are magnifications of (a–d). (a′′,b′′,c′′,d′′) are magnifications of (a′,b′,c′,d′). (e) Quantitative evaluation of Biotin-556 tracer stops/cells in healthy, non-lesional and lesional AD skin. (f,g) Correlation of Cldn-1 immunointensity in SG to Biotin-556 stops/cells (f) and TEWL (g). (e) Mean + SEM. n = 13 per group. *Significant compared to healthy control; #significant compared to non-lesional skin. a.u. arbitrary units. Bars: 50 µm.