Figure 2

Schematic of Flight animal operations. (A) Flow chart of operations from launch through descent of processed tissues. Experimental (n = 10/compartment, 32 wks old at launch) and Validation (n = 10, 16 wks old at launch) mice were loaded into Transporters 2 days before launch, launched on SpaceX Dragon, then transferred into Habitats on the ISS 4 days later (5 mice/compartment). (B) Mice were euthanized after either 21/22 days (Experimental mice) or 37 days (Validation mice) after launch. The indicated tissues were processed for the Validation study. Spleens and livers from 5 of the 10 ISS National Lab Experimental mice were analyzed in the Validation study. *A Vivarium group was not included in the ISS National Lab Experimental study; **MuRF1 KO transgenic mice.