Figure 1
miR-144 repressed gliomas proliferation. (a) The expression of miR-144 were examined in normal brain tissue (n = 12) and glioma tissue (n = 24). (b) The expression of miR-144 were examined in glioma with pathological grade I/II (n = 11), grade III (n = 7) and grade IV (n = 6). (c) The expression of miR-144 was determined in cortical neuron cell line, astrocyte cell line and different glioma cell lines (n = 5). (d) and (e) Different glioma cells were transfected with miR-144 mimic or ASO, and the level of miR-144 was detected (n = 5). (f) U251 cells were seeded in 96-well plates after transfecting miR-144 mimic or control oligonucleotide (Scramble), and un-transfected cells as the negative control (Control). The cell proliferation was evaluated at 24 h, 48 h, 72 h and 96 h (n = 5). (g) U251 cells were seeded in 96-well plates after transfecting miR-144 inhibitor or control oligonucleotide (Scramble), and un-transfected cells as the negative control (Control). The cell proliferation was evaluated at 24 h, 48 h, 72 h and 96 h (n = 5). (h,i) U251 cells overexpressing miR-144 (f) or inhibiting miR-144 (g) were collected and flow cytometry analysis was performed to detect the cell cycle (n = 4). J and (k) U251 cells were transfected miR-144 mimic (h) or miR-144 inhibitor (i) and seeded into 6-well plates for culture. The numbers of cells colonies were count and analyzed (n = 4). Bars represent means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001.
